Human platelet activating factor,PAF ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07929h

Sensitivity

1.17 ng/mL

Detection Range

4.69 ng/mL-300 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

platelet activating factor,PAF

Recovery

The recovery of mouse APOC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	101	94-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	97
	Range %	88-102
1:40	Average %	93
	Range %	85-98
1:80	Average %	103
	Range %	96-107
1:160	Average %	104
	Range %	95-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	PAF

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Characterization of antioxidant/anti-inflammatory properties and apoA-I-containing subpopulations of HDL from family subjects with monogenic low HDL disorders Masakazu Kurita et al,Clinica Chimica Acta,2011 Jun 11;412(13-14):1213-20. The association of MDR1 C3435T and G2677T/A polymorphisms with plasma platelet-activating factor levels and coronary artery disease risk in Turkish population Ayaz G et al,Gene,2013 Jun 28.pii: S0378-1119(13)00803-2. Platelet activating factor contributes to vascular leak in acute dengue infection Jeewandara C et al,PLoS Negl Trop Dis,2015 Feb 3;9(2):e0003459. Secretory phospholipase A2 in the pathogenesis of acute dengue infection Chandima Jeewandara.et al,Immunity, Inflammation and Disease,October

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