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Home / ELISA Kit

Human Plasmin-antiplasmin complex (PAP) ELISA Kit

SKU : BHE13703523

alpha2-Antiplasmin is the principal plasma inhibitor of plasmin, with which it rapidly and irreversibly forms a complex. The simple technique of crossed immunoelectrophoresis against antiserum to alpha2-antiplasmin has been applied to detect -antiplasmin complex in plasma. The presence of this complex is demonstrated in patients in whom overactive fibrinolysis was the only or major contributor to severe haemorrhagic disorders arising spontaneously.
When streptokinase-activated, urokinase-activated, or spontaneously activated human plasmin is mixed with increasing amounts of human antiplasmin, caseinolytic effect decreases much more rapidly than fibrinolytic effect. At concentrations of antiplasmin at which caseinolytic effect completely disappears, considerable fibrinolytic activity persists.

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Mfr.No.	KTE61307
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Plasmin-antiplasmin complex (PAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PAP.
Alternative Names	PAP
Detection Method	Colorimetric
Kit Components	Human Plasmin-antiplasmin complex microplate Human Plasmin-antiplasmin complex standard Human Plasmin-antiplasmin complex detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Plasmin-antiplasmin complex (PAP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PAP. No significant cross-reactivity or interference between Human PAP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	alpha2-Antiplasmin is the principal plasma inhibitor of plasmin, with which it rapidly and irreversibly forms a complex. The simple technique of crossed immunoelectrophoresis against antiserum to alpha2-antiplasmin has been applied to detect -antiplasmin complex in plasma. The presence of this complex is demonstrated in patients in whom overactive fibrinolysis was the only or major contributor to severe haemorrhagic disorders arising spontaneously. When streptokinase-activated, urokinase-activated, or spontaneously activated human plasmin is mixed with increasing amounts of human antiplasmin, caseinolytic effect decreases much more rapidly than fibrinolytic effect. At concentrations of antiplasmin at which caseinolytic effect completely disappears, considerable fibrinolytic activity persists.
Notes	This Human Plasmin-antiplasmin complex (PAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PAP in samples. An antibody specific for PAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Plasmin-antiplasmin complex (PAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PAP in samples. An antibody specific for PAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703523	KTE61307	48 T	$359		Add to Cart
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BHE13703523	KTE61307	96 Tx50	$26209		Add to Cart

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