Human plasmin-antiplasmin complex,PAP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08434h

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

plasmin-antiplasmin complex,PAP

Recovery

The recovery of human CML spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-98
EDTA plasma (n=4)	94	91-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CML in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-95
1:2	Average %	94
	Range %	90-100
1:4	Average %	95
	Range %	91-105
1:8	Average %	94
	Range %	85-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














pg/ml	OD1	OD2	Average	Corrected
4000	2.372	2.252	2.312	2.162
2000	1.775	1.660	1.718	1.568
1000	1.160	1.142	1.151	1.001
500	0.712	0.699	0.706	0.556
250	0.493	0.456	0.475	0.325
125	0.342	0.329	0.336	0.186
62.5	0.264	0.249	0.257	0.107
0	0.153	0.147	0.150	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	PAP

Storage	Store at 2-8°C. Please refer to protocol.

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