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Home / ELISA Kit

Human Plasma Membrane Calcium-transporting Atpase 2 - ATP2B2 ELISA Kit

SKU : BHE12103778

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

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Bioassay Technology Laboratory

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Mfr.No.

E2238Hu

Sensitivity

0.022ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ATP2B2;Plasma membrane calcium ATPase isoform 2;Plasma membrane calcium pump isoform 2;Plasma membrane calcium-transporting ATPase 2;PMCA2

Molecular Weight

136876

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2238Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.3	0.16	4.7
Intra-Assay	2	18	7.7	0.22	2.8
Intra-Assay	3	18	1.3	0.07	5.1

Background

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Protocol

These standard curves of E2238Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.115	2.158	2.086
	2.201
6ng/ml	1.215	1.275	1.203
	1.336
3ng/ml	0.718	0.761	0.689
	0.804
1.5ng/ml	0.556	0.522	0.45
	0.488
0.75ng/ml	0.312	0.289	0.216
	0.266
0ng/ml	0.077	0.072	0
	0.068

Gene ID	491
UniProt ID	Q01814

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103778	E2238Hu	48T	$320		Add to Cart
BHE12103778	E2238Hu	96T	$458		Add to Cart

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