Human placental protein 13,PP13 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E12733h

Alternative Names

lectin, galactoside-binding, soluble, 13, GAL13, PLAC8, PP13, beta-galactoside-binding lectin, galectin 13, galectin-13, placental protein 13

Reactivity

Human

Detection Range

3.9 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Serum, Urine

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human BCL2L11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	102	98-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BCL2L11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	92
	Range %	84-97
1:4	Average %	99
	Range %	95-105
1:8	Average %	97
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9UHV8
Antigen	lectin, galactoside-binding, soluble, 13

Applications

ELISA

Research Area

LGALS13

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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