Human placenta growth factor,PLGF ELISA kit

Human placenta growth factor,PLGF ELISA kit

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Mfr.No.

CSB-E04707h

Sensitivity

0.7 ng/mL

Detection Range

1.67 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

placental growth factor, D12S1900, PGFL, PLGF, PlGF-2, SHGC-10760, placenta growth factor, placental growth factor, vascular endothelial growth factor-related protein, placental growth factor-like

Recovery

The recovery of human FURIN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-100
EDTA plasma (n=4)	99	92-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FURIN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-91
1:2	Average %	96
	Range %	90-100
1:4	Average %	99
	Range %	94-104
1:8	Average %	92
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	1.925	1.852	1.889	1.805
750	1.501	1.476	1.489	1.405
375	1.074	1.004	1.039	0.955
187.5	0.783	0.739	0.761	0.677
94	0.496	0.487	0.492	0.408
47	0.318	0.309	0.314	0.230
23.5	0.233	0.229	0.231	0.147
0	0.087	0.081	0.084	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P49763

Applications	ELISA
Research Area	PGF

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503790	CSB-E04707h	96T	$705	Add to Cart
BHE10503790	CSB-E04707h	5x96T	$3384	Add to Cart
BHE10503790	CSB-E04707h	10x96T	$6697.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.