Human PKLR ELISA Kit PicoKine®

Mfr.No.

EK2257

Sensitivity

<45 pg/ml

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	93.7	187.5	375	750	1,500	3,000	6,000
O.D.	0.031	0.087	0.156	0.365	0.542	0.761	1.413	2.048

Description

Human PKLR ELISA Kit PicoKine® (96 Tests). Quantitate Human PKLR in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 45 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L47-S574

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human PKLR

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	185	335	2366	185	267	1851
Standard deviation	7.25	13.97	57.97	8.94	18.42	116.8
CV(%)	3.9%	4.2%	2.5%	4.8%	6.9%	6.3%

Background

Pyruvate kinase PKLR is an enzyme that in humans is encoded by the PKLR gene. The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	185	168	186	177	179	13.75	7.7%
Sample 2	335	263	278	311	297	11.07	3.7%
Sample 3	2366	1875	2286	2162	2172	144.02	6.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5313
Gene Name	PKLR
UniProt ID	P30613

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001807	EK2257	96 wells/kit	$750		Add to Cart

Human PKLR ELISA Kit PicoKine®

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