Human Pituitary Adenylate Cyclase-activating Polypeptide

Mfr.No.

E2818Hu

Sensitivity

6.87ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ADCYAP1;PACAP;PACAP27;PACAP-27;PACAP-38;PACAP38);PACAP-related Peptide;Pituitary adenylate cyclase-activating polypeptide;Pituitary adenylate cyclase-activating polypeptide 27;Pituitary adenylate cyclase-activating polypeptide 38;PRP-48

Molecular Weight

18835

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2818Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	396.84	23.65	5.96
Intra-Assay	2	18	1042.87	34.88	3.34
Intra-Assay	3	18	471.09	26.8	5.69

Background

Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway. In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion (By similarity). Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells (By similarity).

Protocol

These standard curves of E2818Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.336	2.379	2.35
	2.421
800ng/L	1.33	1.271	1.243
	1.213
400ng/L	0.782	0.744	0.716
	0.707
200ng/L	0.409	0.418	0.389
	0.426
100ng/L	0.216	0.171	0.143
	0.127
0ng/L	0.036	0.028	0
	0.021

Gene ID	116
UniProt ID	P18509

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104355	E2818Hu	48T	$320		Add to Cart
BHE12104355	E2818Hu	96T	$458		Add to Cart

Human Pituitary Adenylate Cyclase-activating Polypeptide - ADCYAP1 ELISA Kit

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