Human PIP(Prolactin-inducible protein) ELISA Kit

Mfr.No.

EH2124

Description

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PIP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with PIP bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of PIP in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Alternative Names

PIP ELISA Kit, GCDFP-15 ELISA Kit, GCDFP15 ELISA Kit, GPIP4 ELISA Kit, gp17 ELISA Kit, Secretory actin-binding protein ELISA Kit, SABP ELISA Kit, Gross cystic disease fluid protein 15 ELISA Kit, Prolactin-induced protein ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

31.25-2000pg/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of PIP and the recovery rates were calculated by comparing the measured value to the expected amount of PIP in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	88-105	96
EDTA plasma(n=5)	88-103	95
heparin plasma(n=5)	87-99	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	90-99%	85-92%	85-103%
EDTA plasma(n=5)	93-100%	85-101%	83-100%
heparin plasma(n=5)	82-100%	87-98%	81-94%

Sensitivity

< 18.75pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

PIP/GCDFP-15/GCDFP15/GPIP4/gp17/Secretory actin-binding protein(SABP)/Gross cystic disease fluid protein 15/Prolactin-induced protein/

Notes

For Research Use Only

UniProt ID	P12273
Antigen	PIP

Applications	ELISA
Applications Notes	PIP ELISA Kit allows for the in vitro quantitative determination of PIP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

Reference	Prolactin Inducible Protein, but Not Prolactin, Is Present in Human Tears, Is Involved in Tear Film Quality, and Influences Evaporative Dry Eye Disease IF: 5.058 Journal: Frontiers in Medicine Cited Date: 2022-07-22

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SKU	Supplier No.	Size	Your price	Quantity
BHE10801467	EH2124	96T	$400		Add to Cart

Human PIP (Prolactin-inducible protein) ELISA Kit

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