Human Pikachurin(EGFLAM) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL007478HU

Alternative Names

EGF-like, fibronectin type III and laminin G domains, AGRINL, AGRNL, FLJ39155, pikachurin

Reactivity

Human

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat NEFL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-94
EDTA plasma (n=4)	99	91-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat NEFL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	85-98
1:2	Average %	107
	Range %	97-111
1:4	Average %	90
	Range %	83-94
1:8	Average %	97
	Range %	91-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

7.81 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q63HQ2
Antigen	EGF-like, fibronectin type III and laminin G domains

Applications

ELISA

Research Area

EGFLAM

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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