Human PIK3CA(Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform) ELISA Kit

Mfr.No.

EH1594

Alternative Names

PIK3CA ELISA Kit, Phosphatidylinositol-4 ELISA Kit, 5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA Kit, PI3-kinase subunit alpha ELISA Kit, PI3K-alpha ELISA Kit, PI3Kalpha ELISA Kit, PtdIns-3-kinase subunit alpha ELISA Kit, Phosphatidylinositol-4 ELISA Kit, 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha ELISA Kit, PtdIns-3-kinase subunit p110-alpha ELISA Kit, p110alpha ELISA Kit, Phosphoinositide-3-kinase catalytic alpha polypeptide ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of PIK3CA and the recovery rates were calculated by comparing the measured value to the expected amount of PIK3CA in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	88-103	95
EDTA plasma(n=5)	86-103	95
heparin plasma(n=5)	88-105	97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIK3CA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	88-104%	92-104%	86-99%
EDTA plasma(n=5)	83-94%	83-99%	82-88%
heparin plasma(n=5)	84-98%	80-99%	85-100%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

PIK3CA/Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform/PI3-kinase subunit alpha/PI3K-alpha/PI3Kalpha/PtdIns-3-kinase subunit alpha/Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha/PtdIns-3-kinase subunit p110-alpha/p110alpha/Phosphoinositide-3-kinase catalytic alpha polypeptide

Notes

For Research Use Only

UniProt ID	P42336
Antigen	PIK3CA

Applications	ELISA
Applications Notes	PIK3CA ELISA Kit allows for the in vitro quantitative determination of PIK3CA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10801605	EH1594	96T	$400		Add to Cart

Human PIK3CA (Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform) ELISA Kit

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