Matrices listed below were spiked with certain level of PIK3AP1 and the recovery rates were calculated by comparing the measured value to the expected amount of PIK3AP1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
94
EDTA plasma(n=5)
89-101
95
heparin plasma(n=5)
85-104
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIK3AP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-103%
91-102%
90-103%
EDTA plasma(n=5)
88-101%
82-92%
82-101%
heparin plasma(n=5)
80-98%
80-94%
87-96%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q6ZUJ8
Applications
ELISA
Applications Notes
PIK3AP1 ELISA Kit allows for the in vitro quantitative determination of PIK3AP1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PIK3AP1 (Phosphoinositide 3-kinase adapter protein 1) ELISA Kit