Human pigment epithelium-derived factor,PEDF ELISA Kit

Mfr.No.

CSB-E08818h

Sensitivity

0.039 ng/mL

Reactivity

Human

Sample Type

Serum, Cell culture supernatants, Urine, Tissue homogenates

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

pigment epithelium-derived factor,PEDF, EPC-1, PEDF, PIG35, pigment epithelium-derived factor, proliferation-inducing protein 35, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor)

Recovery

The recovery of dog IL33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	88-95
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-89
1:2	Average %	102
	Range %	97-107
1:4	Average %	89
	Range %	85-93
1:8	Average %	96
	Range %	93-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.929	2.968	2.949	2.853
750	2.406	2.300	2.353	2.257
375	1.631	1.645	1.638	1.542
187.5	0.915	0.935	0.925	0.829
93.75	0.506	0.500	0.503	0.407
46.88	0.254	0.275	0.265	0.169
23.44	0.141	0.152	0.147	0.051
0	0.094	0.098	0.096	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P36955
Antigen	pigment epithelium-derived factor,PEDF

Applications	ELISA
Research Area	PEDF

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Proliferative and inflammatory factors in the vitreous of patients with proliferative diabetic retinopathy. Chernykh VV et al,Indian J Ophthalmol,2015 Jan;63(1):33-6. PEDF Inhibits the Activation of NLRP3 Inflammasome in Hypoxia Cardiomyocytes through PEDF Receptor/Phospholipase A2 Zhongxin Zhou.et al,Int. J. Mol. Sci,Dec 12 Splicing factor PRPF31 retinitis pigmentosa (RP11) is caused by disrupted alternative splicing programmes for genes implicated in pre-mRNA splicing, cellular adhesion and ciliogenesis Buskin, A.et al,Nature Communications,43326

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SKU	Supplier No.	Size	Your price
BHE10503784	CSB-E08818h	96T	$695	Add to Cart
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BHE10503784	CSB-E08818h	10x96T	$6602.50	Add to Cart

Human pigment epithelium-derived factor,PEDF ELISA Kit

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