Human Phospholipase D1 - PLD1 ELISA Kit

Mfr.No.

E2640Hu

Sensitivity

7.96ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Phospholipase D1 (also known as PLD1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Choline phosphatase 1;CVDD;hPLD1;Phosphatidylcholine-hydrolyzing phospholipase D1;Phospholipase D1;PLD 1;PLD1

Molecular Weight

124184

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2640Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	367.32	19.05	5.19
Intra-Assay	2	18	530.04	25.09	4.73
Intra-Assay	3	18	1111.3	46.33	4.17

Background

Phospholipase D1 (PLD1) is an enzyme that in humans is encoded by the PLD1 gene, though analogues are found in plants, fungi, prokaryotes, and even viruses.

Protocol

These standard curves of E2640Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.113	2.191	2.162
	2.269
800ng/L	1.415	1.361	1.332
	1.308
400ng/L	0.631	0.633	0.604
	0.635
200ng/L	0.44	0.384	0.355
	0.328
100ng/L	0.15	0.148	0.119
	0.147
0ng/L	0.03	0.029	0
	0.028

Gene ID	5337
UniProt ID	Q13393

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104178	E2640Hu	48T	$320		Add to Cart
BHE12104178	E2640Hu	96T	$458		Add to Cart

Human Phospholipase D1 - PLD1 ELISA Kit

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