Human phosphoglycerate mutase 2,PGAM2 ELISA Kit

Mfr.No.

CSB-E11282h

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

phosphoglycerate mutase 2 (muscle), GSD10, MGC88743, PGAM-M, PGAMM, BPG-dependent PGAM 2, phosphoglycerate mutase 2

Recovery

The recovery of rat IL34 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	107	103-110
EDTA plasma (n=4)	86	83-89

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL34 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-93
1:2	Average %	107
	Range %	103-110
1:4	Average %	85
	Range %	81-89
1:8	Average %	89
	Range %	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P15259

Applications	ELISA
Research Area	PGAM2

Storage	Store at 2-8°C. Please refer to protocol.

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