Human Phosphoglycerate Mutase 1

Mfr.No.

E5867Hu

Sensitivity

7.16ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

BPG-dependent PGAM 1;HEL-S-35;PGAM1;PGAMA;PGAM-B;Phosphoglycerate mutase 1;Phosphoglycerate mutase isozyme B

Molecular Weight

28804

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5867Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	379.05	22.13	5.84
Intra-Assay	2	18	647.1	32.49	5.02
Intra-Assay	3	18	180.37	9.85	5.46

Background

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.

Protocol

These standard curves of E5867Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.03	2.162	2.116
	2.295
800ng/L	1.897	1.927	1.881
	1.958
400ng/L	1.222	1.167	1.121
	1.112
200ng/L	0.67	0.706	0.66
	0.742
100ng/L	0.438	0.427	0.381
	0.417
0ng/L	0.042	0.046	0
	0.05

Gene ID	5223
UniProt ID	P18669

Research Area	Signal Transduction; Cancer; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107229	E5867Hu	48T	$320		Add to Cart
BHE12107229	E5867Hu	96T	$458		Add to Cart

Human Phosphoglycerate Mutase 1 - PGAM1 ELISA Kit

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