Human phosphoglycerate kinase 1 (PGK1) ELISA kit

Human phosphoglycerate kinase 1 (PGK1) ELISA kit

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Mfr.No.

CSB-E17849h

Alternative Names

phosphoglycerate kinase 1, RP4-570L12.1, MGC117307, MGC142128, MGC8947, MIG10, PGKA, cell migration-inducing gene 10 protein, primer recognition protein 2

Reactivity

Human

Detection Range

28 pg/mL-1800 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat COL ? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	104	99-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat COL ? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-105
1:2	Average %	96
	Range %	92-101
1:4	Average %	92
	Range %	87-96
1:8	Average %	87
	Range %	82-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.221	2.120	2.171	2.019
50	1.441	1.340	1.391	1.239
25	0.825	0.804	0.815	0.663
12.5	0.519	0.530	0.525	0.373
6.25	0.335	0.324	0.330	0.178
3.12	0.240	0.251	0.246	0.094
1.56	0.196	0.192	0.194	0.042
0	0.152	0.151	0.152	?

Species

Homo sapiens

Sensitivity

7 pg/mL

Limit Of Detection

450 nm

UniProt ID	P00558
Antigen	Phosphoglycerate Kinase 1

Applications

ELISA

Research Area

PGK1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503765	CSB-E17849h	96T	$790	Add to Cart
BHE10503765	CSB-E17849h	5x96T	$3792	Add to Cart
BHE10503765	CSB-E17849h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.