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Home / ELISA Kit

Human Phosphofructokinase-platelet (PFKP) ELISA Kit

SKU : BHE13703458

Phosphofructokinase, platelet, also known as PFKP is the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose 6-phosphate to fructose 1,6-bisphosphate and is a key regulatory enzyme in glycolysis.
The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM) and liver (PFKL) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.

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Mfr.No.	KTE61242
Description	This Human Phosphofructokinase-platelet (PFKP) ELISA Kit employs a two-site sandwich ELISA to quantitate PFKP.
Alternative Names	PFKP; RP11-298E9.2; FLJ40226; PFK-C; PFKF; Phosphofructokinase; platelet type
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Phosphofructokinase-platelet microplate Human Phosphofructokinase-platelet standard Human Phosphofructokinase-platelet detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Phosphofructokinase-platelet (PFKP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PFKP. No significant cross-reactivity or interference between Human PFKP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Phosphofructokinase, platelet, also known as PFKP is the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose 6-phosphate to fructose 1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM) and liver (PFKL) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.
Notes	This Human Phosphofructokinase-platelet (PFKP) ELISA Kit employs a two-site sandwich ELISA to quantitate PFKP in samples. An antibody specific for PFKP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFKP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	5214
Antigen	PFKP
Tag	PFKP
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Phosphofructokinase-platelet (PFKP) ELISA Kit employs a two-site sandwich ELISA to quantitate PFKP in samples. An antibody specific for PFKP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFKP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703458	KTE61242	48 T	$339		Add to Cart
BHE13703458	KTE61242	96 T	$589		Add to Cart
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BHE13703458	KTE61242	96 Tx50	$24569		Add to Cart

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