Matrices listed below were spiked with certain level of Phosphatidylinositol Trisphosphate and the recovery rates were calculated by comparing the measured value to the expected amount of Phosphatidylinositol Trisphosphate in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
94
EDTA plasma(n=5)
87-100
93
heparin plasma(n=5)
88-100
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Phosphatidylinositol Trisphosphate and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-103%
85-102%
86-103%
EDTA plasma(n=5)
85-94%
87-100%
92-100%
heparin plasma(n=5)
85-98%
85-97%
92-100%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Phosphatidylinositol Trisphosphate ELISA Kit allows for the in vitro quantitative determination of Phosphatidylinositol Trisphosphate concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of Human Phosphatidylinositol Trisphosphate ELISA Kit