Human Phosphatidylinositol-glycan-specific phospholipase D(GPLD1) ELISA kit

Human Phosphatidylinositol-glycan-specific phospholipase D(GPLD1) ELISA kit

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Mfr.No.

CSB-EL009721HU

Sensitivity

0.78 ng/mL

Detection Range

3.12 ng/mL-200 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

glycosylphosphatidylinositol specific phospholipase D1, GPIPLD, GPIPLDM, MGC22590, PIGPLD, PIGPLD1, GPI-specific phospholipase D, glycoprotein phospholipase D, glycosylphosphatIdylinositol-specific phospholipase D, glycosylphosphatidylinositol specific phos

Recovery

The recovery of human MELK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-99
EDTA plasma (n=4)	95	89-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MELK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	86
	Range %	80-93
1:4	Average %	96
	Range %	90-101
1:8	Average %	100
	Range %	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.776	2.863	2.820	2.671
5	2.121	1.995	2.058	1.909
2.5	1.142	1.241	1.192	1.043
1.25	0.705	0.733	0.719	0.570
0.625	0.393	0.387	0.390	0.241
0.312	0.296	0.284	0.290	0.141
0.156	0.238	0.245	0.242	0.093
0	0.150	0.148	0.149	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P80108

Applications	ELISA
Research Area	GPLD1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503759	CSB-EL009721HU	96T	$695	Add to Cart
BHE10503759	CSB-EL009721HU	5x96T	$3336	Add to Cart
BHE10503759	CSB-EL009721HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.