Matrices listed below were spiked with certain level of Phosphatidylinositol Disphosphate and the recovery rates were calculated by comparing the measured value to the expected amount of Phosphatidylinositol Disphosphate in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-102
97
EDTA plasma(n=5)
89-104
98
heparin plasma(n=5)
89-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Phosphatidylinositol Disphosphate and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-95%
85-95%
86-101%
EDTA plasma(n=5)
90-101%
85-100%
85-99%
heparin plasma(n=5)
86-94%
87-100%
85-96%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Phosphatidylinositol Disphosphate ELISA Kit allows for the in vitro quantitative determination of Phosphatidylinositol Disphosphate concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of Human Phosphatidylinositol Disphosphate ELISA Kit