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Home / ELISA Kit

Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2) ELISA Kit

SKU : BHE13703385

Prex2 was abundantly expressed in mouse brain and lung, with lower expression in liver, thymus, and spleen. In brain, highest expression was in cerebellum, with lower expression in frontal cortex, striatum, amygdala and hippocampus. In situ hybridization showed that Prex2 mRNA was highly restricted to cerebellum, with expression in Purkinje cells, including dendrites.Expression of PREX2 in PAE cells induced this 'activated Rac' morphology, which was reduced by inhibition of PI3K signaling and enhanced by PDGF stimulation. PREX2-mediated GTP loading onto RAC was synergistically stimulated by PI3K and G protein beta-1 (GNB1)/gamma-2 (GNG2) subunits. Direct binding of phosphatidylinositol 3,4,5-trisphosphate to PREX2 was sufficient to stimulate PREX2 RAC-guanine nucleotide exchange factor activity.

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Mfr.No.	KTE61169
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2) ELISA Kit employs a two-site sandwich ELISA to quantitate PREX2.
Alternative Names	PREX2; DEP.2; DEPDC2; P-REX2; 6230420N16Rik; DEP domain containing 2; PtdIns(3;4;5)-dependent Rac exchanger 2; phosphatidylinositol 3;4;5-trisphosphate-dependent RAC exchanger 2
Detection Method	Colorimetric
Kit Components	Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein microplate Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein standard Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PREX2. No significant cross-reactivity or interference between Human PREX2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Prex2 was abundantly expressed in mouse brain and lung, with lower expression in liver, thymus, and spleen. In brain, highest expression was in cerebellum, with lower expression in frontal cortex, striatum, amygdala and hippocampus. In situ hybridization showed that Prex2 mRNA was highly restricted to cerebellum, with expression in Purkinje cells, including dendrites.Expression of PREX2 in PAE cells induced this 'activated Rac' morphology, which was reduced by inhibition of PI3K signaling and enhanced by PDGF stimulation. PREX2-mediated GTP loading onto RAC was synergistically stimulated by PI3K and G protein beta-1 (GNB1)/gamma-2 (GNG2) subunits. Direct binding of phosphatidylinositol 3,4,5-trisphosphate to PREX2 was sufficient to stimulate PREX2 RAC-guanine nucleotide exchange factor activity.
Notes	This Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2) ELISA Kit employs a two-site sandwich ELISA to quantitate PREX2 in samples. An antibody specific for PREX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPREX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PREX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PREX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	80243

Applications	ELISA
Applications Notes	This Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2) ELISA Kit employs a two-site sandwich ELISA to quantitate PREX2 in samples. An antibody specific for PREX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPREX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PREX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PREX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703385	KTE61169	48 T	$339		Add to Cart
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BHE13703385	KTE61169	96 Tx50	$24569		Add to Cart

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