Human Phosphatidylethanolamine Antibody(IgM) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E15752h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

Phosphatidylethanolamine Antibody(IgM)

Recovery

The recovery of rat MIP-1? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	99-108
EDTA plasma (n=4)	95	91-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-98
1:2	Average %	96
	Range %	91-104
1:4	Average %	99
	Range %	96-105
1:8	Average %	95
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IgM

Storage	Store at 2-8°C. Please refer to protocol.

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