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Home / ELISA Kit

Human Phosphatidate phosphatase LPIN1 (LPIN1) ELISA Kit

SKU : BHE13704003

Lipin-1 has phosphatidate phosphatase activity.This gene represents a candidate gene for human lipodystrophy, characterized by loss of body fat, fatty liver, hypertriglyceridemia, and insulin resistance. Mouse studies suggest that this gene functions during normal adipose tissue development and may also play a role in human triglyceride metabolism.
Database analysis identified the human LPIN1 gene, as well as the human and mouse LPIN2 and LPIN3 genes. Consistent with the observed reduction of adipose tissue mass in fld mice, wildtype Lpin1 mRNA was expressed at high levels in adipose tissue and was induced during differentiation of 3T3-L2 preadipocytes. The results indicated that lipin is required for normal adipose tissue development, and provided a candidate gene for human lipodystrophy.

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Mfr.No.	KTE61788
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Phosphatidate phosphatase LPIN1 (LPIN1) ELISA Kit employs a two-site sandwich ELISA to quantitate LPIN1.
Alternative Names	LPIN1; DKFZp781P1796; KIAA0188; PAP1;
Detection Method	Colorimetric
Kit Components	Human Phosphatidate phosphatase LPIN1 microplate Human Phosphatidate phosphatase LPIN1 standard Human Phosphatidate phosphatase LPIN1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Phosphatidate phosphatase LPIN1 (LPIN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LPIN1. No significant cross-reactivity or interference between Human LPIN1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Lipin-1 has phosphatidate phosphatase activity.This gene represents a candidate gene for human lipodystrophy, characterized by loss of body fat, fatty liver, hypertriglyceridemia, and insulin resistance. Mouse studies suggest that this gene functions during normal adipose tissue development and may also play a role in human triglyceride metabolism. Database analysis identified the human LPIN1 gene, as well as the human and mouse LPIN2 and LPIN3 genes. Consistent with the observed reduction of adipose tissue mass in fld mice, wildtype Lpin1 mRNA was expressed at high levels in adipose tissue and was induced during differentiation of 3T3-L2 preadipocytes. The results indicated that lipin is required for normal adipose tissue development, and provided a candidate gene for human lipodystrophy.
Notes	This Human Phosphatidate phosphatase LPIN1 (LPIN1) ELISA Kit employs a two-site sandwich ELISA to quantitate LPIN1 in samples. An antibody specific for LPIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPIN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	23175

Applications	ELISA
Applications Notes	This Human Phosphatidate phosphatase LPIN1 (LPIN1) ELISA Kit employs a two-site sandwich ELISA to quantitate LPIN1 in samples. An antibody specific for LPIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPIN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704003	KTE61788	48 T	$339		Add to Cart
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BHE13704003	KTE61788	96 Tx50	$24569		Add to Cart

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