Human Phosphatase and actin regulator 1(PHACTR1) ELISA kit

Mfr.No.

CSB-EL017880HU

Alternative Names

phosphatase and actin regulator 1, RP1-257A7.1, KIAA1733, MGC126575, MGC126577, RPEL, RPEL1, dJ257A7.2, RPEL repeat containing 1

Reactivity

Human

Detection Range

23.5 pg/mL-1500 pg/mL

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-90
EDTA plasma (n=4)	104	100-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	83
	Range %	80-86
1:4	Average %	95
	Range %	80-90
1:8	Average %	102
	Range %	98-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

5.86 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9C0D0
Antigen	phosphatase and actin regulator 1

Applications

ELISA

Research Area

PHACTR1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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