Human Phenylethanolamine N-methyltransferase

Mfr.No.

E5859Hu

Sensitivity

9.75ng/L

Detection Range

20-3500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Noradrenaline N-methyltransferase;PENT;Phenylethanolamine N-methyltransferase;PNMT;PNMTase

Molecular Weight

30855

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5859Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	697.18	35.41	5.08
Intra-Assay	2	18	1271.37	32.2	2.53
Intra-Assay	3	18	447.65	18.85	4.21

Background

Phenylethanolamine N-methyltransferase (PNMT) is an enzyme found primarily in the adrenal medulla that converts norepinephrine (noradrenaline) to epinephrine (adrenaline). It is also expressed in small groups of neurons in the human brain and in selected populations of cardiomyocytes.

Protocol

These standard curves of E5859Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.37	2.254	2.238
	2.137
1000ng/L	1.349	1.302	1.287
	1.256
500ng/L	0.594	0.59	0.575
	0.586
250ng/L	0.304	0.338	0.323
	0.373
125ng/L	0.147	0.157	0.142
	0.167
0ng/L	0.014	0.015	0
	0.016

Gene ID	5409
UniProt ID	P11086

Research Area	Neuroscience; Cardiovascular; Cancer; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107221	E5859Hu	48T	$320		Add to Cart
BHE12107221	E5859Hu	96T	$458		Add to Cart

Human Phenylethanolamine N-methyltransferase - PNMT ELISA Kit

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