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Home / ELISA Kit

Human PHD finger protein 7 (PHF7) ELISA Kit

SKU : BHE13703437

Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, PHF7 is expressed in Sertoli cells but not germ cells. However, this gene is not expressed in a patient who exhibited spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest is an interruption of germ cell differentiation that may result in oligospermia or azoospermia. The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. Thus this protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis.

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Mfr.No.	KTE61221
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human PHD finger protein 7 (PHF7) ELISA Kit employs a two-site sandwich ELISA to quantitate PHF7.
Alternative Names	PHF7; DKFZp434L1850; HSPC045; HSPC226; MGC26088; NYD-SP6;
Detection Method	Colorimetric
Kit Components	Human PHD finger protein 7 microplate Human PHD finger protein 7 standard Human PHD finger protein 7 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human PHD finger protein 7 (PHF7) ELISA Kit has high sensitivity and excellent specificity for detection of Human PHF7. No significant cross-reactivity or interference between Human PHF7 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, PHF7 is expressed in Sertoli cells but not germ cells. However, this gene is not expressed in a patient who exhibited spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest is an interruption of germ cell differentiation that may result in oligospermia or azoospermia. The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. Thus this protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis.
Notes	This Human PHD finger protein 7 (PHF7) ELISA Kit employs a two-site sandwich ELISA to quantitate PHF7 in samples. An antibody specific for PHF7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPHF7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHF7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHF7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	51533

Applications	ELISA
Applications Notes	This Human PHD finger protein 7 (PHF7) ELISA Kit employs a two-site sandwich ELISA to quantitate PHF7 in samples. An antibody specific for PHF7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPHF7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHF7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHF7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703437	KTE61221	48 T	$339		Add to Cart
BHE13703437	KTE61221	96 T	$589		Add to Cart
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BHE13703437	KTE61221	96 Tx50	$24569		Add to Cart

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