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Home / ELISA Kit

Human Pescadillo homolog (PES1) ELISA Kit

SKU : BHE13703463

PES1 is abnormally elevated in malignant tumors of astrocytic origin. It is a strongly conserved gene containing a BRCT domain that is essential for the activity of this gene product. The gene plays a crucial role in cell proliferation and may be necessary for oncogenic transformation and tumor progression.The deduced 588-amino acid PES1 protein is 74% identical to the zebrafish sequence. The authors noted the presence of a highly acidic region at the C terminus and that the protein has an alpha-helix structure. Immunoblot analysis showed that PES1 is expressed as an unmodified 72-kD protein and a SUMO1-modified 92-kD protein. In situ hybridization analysis revealed wide and high expression of Pes1 in embryonic mouse brain and spinal cord. Pes1 expression was upregulated in malignant, late-passage mouse astrocytes.

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Mfr.No.	KTE61247
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Pescadillo homolog (PES1) ELISA Kit employs a two-site sandwich ELISA to quantitate PES1.
Alternative Names	PES1; PES; pescadillo homolog 1; containing BRCT domain
Detection Method	Colorimetric
Kit Components	Human Pescadillo homolog microplate Human Pescadillo homolog standard Human Pescadillo homolog detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Pescadillo homolog (PES1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PES1. No significant cross-reactivity or interference between Human PES1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PES1 is abnormally elevated in malignant tumors of astrocytic origin. It is a strongly conserved gene containing a BRCT domain that is essential for the activity of this gene product. The gene plays a crucial role in cell proliferation and may be necessary for oncogenic transformation and tumor progression.The deduced 588-amino acid PES1 protein is 74% identical to the zebrafish sequence. The authors noted the presence of a highly acidic region at the C terminus and that the protein has an alpha-helix structure. Immunoblot analysis showed that PES1 is expressed as an unmodified 72-kD protein and a SUMO1-modified 92-kD protein. In situ hybridization analysis revealed wide and high expression of Pes1 in embryonic mouse brain and spinal cord. Pes1 expression was upregulated in malignant, late-passage mouse astrocytes.
Notes	This Human Pescadillo homolog (PES1) ELISA Kit employs a two-site sandwich ELISA to quantitate PES1 in samples. An antibody specific for PES1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPES1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PES1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PES1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	23481

Applications	ELISA
Applications Notes	This Human Pescadillo homolog (PES1) ELISA Kit employs a two-site sandwich ELISA to quantitate PES1 in samples. An antibody specific for PES1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPES1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PES1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PES1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703463	KTE61247	48 T	$339		Add to Cart
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