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Home / ELISA Kit

Human Persephin (PSPN) ELISA Kit

SKU : BHE13703295

Persephin is a neurotrophic factor, belonging to the GDNF family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease.
The encoded protein has amino acid similarity to its other family members, glial cell line-derived neurotrophic factor and neurturin. This gene product promotes the survival of ventral midbrain dopaminergic neurons in culture and prevents their degeneration after 6-hydroxydopamine treatment in vivo.Like GDNF, PSP also had effects on kidney development, as evidenced by its ability to promote ureteric bud branching.

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Mfr.No.	KTE61079
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Persephin (PSPN) ELISA Kit employs a two-site sandwich ELISA to quantitate PSPN.
Alternative Names	PSPN; PSP;
Detection Method	Colorimetric
Kit Components	Human Persephin microplate Human Persephin standard Human Persephin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Persephin (PSPN) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSPN. No significant cross-reactivity or interference between Human PSPN and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Persephin is a neurotrophic factor, belonging to the GDNF family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. The encoded protein has amino acid similarity to its other family members, glial cell line-derived neurotrophic factor and neurturin. This gene product promotes the survival of ventral midbrain dopaminergic neurons in culture and prevents their degeneration after 6-hydroxydopamine treatment in vivo.Like GDNF, PSP also had effects on kidney development, as evidenced by its ability to promote ureteric bud branching.
Notes	This Human Persephin (PSPN) ELISA Kit employs a two-site sandwich ELISA to quantitate PSPN in samples. An antibody specific for PSPN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSPN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSPN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSPN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5623

Applications	ELISA
Applications Notes	This Human Persephin (PSPN) ELISA Kit employs a two-site sandwich ELISA to quantitate PSPN in samples. An antibody specific for PSPN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSPN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSPN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSPN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703295	KTE61079	48 T	$339		Add to Cart
BHE13703295	KTE61079	96 T	$589		Add to Cart
BHE13703295	KTE61079	96 Tx5	$2629		Add to Cart
BHE13703295	KTE61079	96 Tx50	$24569		Add to Cart

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