Human peroxisome proliferative activated receptor gama coactivator 1 alpha,PPARGC1 ELISA Kit

Mfr.No.

CSB-E11761h

Alternative Names

peroxisome proliferator-activated receptor gamma, coactivator 1 alpha, LEM6, PGC-1(alpha), PGC-1v, PGC1, PGC1A, PPARGC1, PPAR gamma coactivator variant form, PPAR gamma coactivator-1, ligand effect modulator-6, peroxisome proliferative activated receptor, gamma, coactivat

Reactivity

Human

Detection Range

125 pg/mL-8000 pg/mL

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse IL-1? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-99
EDTA plasma (n=4)	93	88-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-95
1:2	Average %	93
	Range %	88-98
1:4	Average %	96
	Range %	94-102
1:8	Average %	94
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2000

2.758

2.641

2.700

2.586

1000

1.438

1.587

1.513

1.399

500

0.833

0.869

0.851

0.737

250

0.474

0.477

0.476

0.362

125

0.350

0.375

0.363

0.249

62.5

0.267

0.253

0.260

0.146

31.25

0.165

0.186

0.176

0.062

0.119

0.108

0.114

Species

Homo sapiens

Sensitivity

31.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9UBK2
Antigen	peroxisome proliferator-activated receptor gamma, coactivator 1 alpha

Applications

ELISA

Research Area

PPARGC1A

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503731	CSB-E11761h	96T	$695	Add to Cart
BHE10503731	CSB-E11761h	5x96T	$3336	Add to Cart
BHE10503731	CSB-E11761h	10x96T	$6602.50	Add to Cart

Human peroxisome proliferative activated receptor gama coactivator 1 alpha,PPARGC1 ELISA Kit

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