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Home / ELISA Kit

Human Peroxisomal testis-specific protein 1 (PXT1) ELISA Kit

SKU : BHE13703241

Genes reported to be crucial for spermatogenesis are often exclusively expressed in the testis. The putative amino acid sequence encoded by the cDNA of the Pxt1 gene contains a conserved Asn-His-Leu (NHL)-motif at its C-terminal end, which is characteristic for peroxisomal proteins. Pxt1-EGFP fusion protein is co-localized with known peroxisomal marker proteins in transfected NIH3T3 cells. The peroxisomal targeting signal NHL is functional and responsible for the correct subcellular localization of the Pxt1-EGFP fusion protein. In male germ cells peroxisomes were reported only in spermatogonia. The Pxt1 gene is so far the first gene coding for a putative peroxisomal protein which is expressed in later steps of spermatogenesis, namely in pachytene spermatocytes.

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Mfr.No.	KTE61025
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Peroxisomal testis-specific protein 1 (PXT1) ELISA Kit employs a two-site sandwich ELISA to quantitate PXT1.
Alternative Names	PXT1; MGC129569; STEPP; small testis-specific peroxisomal protein
Detection Method	Colorimetric
Kit Components	Human Peroxisomal testis-specific protein 1 microplate Human Peroxisomal testis-specific protein 1 standard Human Peroxisomal testis-specific protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Peroxisomal testis-specific protein 1 (PXT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PXT1. No significant cross-reactivity or interference between Human PXT1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Genes reported to be crucial for spermatogenesis are often exclusively expressed in the testis. The putative amino acid sequence encoded by the cDNA of the Pxt1 gene contains a conserved Asn-His-Leu (NHL)-motif at its C-terminal end, which is characteristic for peroxisomal proteins. Pxt1-EGFP fusion protein is co-localized with known peroxisomal marker proteins in transfected NIH3T3 cells. The peroxisomal targeting signal NHL is functional and responsible for the correct subcellular localization of the Pxt1-EGFP fusion protein. In male germ cells peroxisomes were reported only in spermatogonia. The Pxt1 gene is so far the first gene coding for a putative peroxisomal protein which is expressed in later steps of spermatogenesis, namely in pachytene spermatocytes.
Notes	This Human Peroxisomal testis-specific protein 1 (PXT1) ELISA Kit employs a two-site sandwich ELISA to quantitate PXT1 in samples. An antibody specific for PXT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	222659

Applications	ELISA
Applications Notes	This Human Peroxisomal testis-specific protein 1 (PXT1) ELISA Kit employs a two-site sandwich ELISA to quantitate PXT1 in samples. An antibody specific for PXT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703241	KTE61025	48 T	$339		Add to Cart
BHE13703241	KTE61025	96 T	$589		Add to Cart
BHE13703241	KTE61025	96 Tx5	$2629		Add to Cart
BHE13703241	KTE61025	96 Tx50	$24569		Add to Cart

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