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Home / ELISA Kit

Human Peroxisomal membrane protein 4 (PXMP4) ELISA Kit

SKU : BHE13703243

Pxmp4, which encodes a 24-kDa peroxisomal integral membrane protein of unknown function. Pxmp4 was originally isolated as a 24-kDa polypeptide from rat liver peroxisome membranes (36). As increased message for Pxmp4 is associated with the NOD allele that confers weaker NKT cell stimulatory capacity, one possibility is that Pxmp4 contributes to the biosynthesis of a nonstimulatory etherphospholipid ligand for CD1d. Pxmp4 may be a component in a fatty acid degradation pathway that catabolizes stimulatory CD1d ligands. Peroxisomes play a critical role in the - and -oxidation of very long chain fatty acids. Pex19 associates with Pxmp4 during or immediately after its translation by free ribosomes. Peroxisomal membrane insertion of the Pxmp4-Pex19 complex is dependent on Pex3p.

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Mfr.No.	KTE61027
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Peroxisomal membrane protein 4 (PXMP4) ELISA Kit employs a two-site sandwich ELISA to quantitate PXMP4.
Alternative Names	PXMP4; PMP24; 24 kDa peroxisomal intrinsic membrane protein; peroxisomal membrane protein 4
Detection Method	Colorimetric
Kit Components	Human Peroxisomal membrane protein 4 microplate Human Peroxisomal membrane protein 4 standard Human Peroxisomal membrane protein 4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Peroxisomal membrane protein 4 (PXMP4) ELISA Kit has high sensitivity and excellent specificity for detection of Human PXMP4. No significant cross-reactivity or interference between Human PXMP4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Pxmp4, which encodes a 24-kDa peroxisomal integral membrane protein of unknown function. Pxmp4 was originally isolated as a 24-kDa polypeptide from rat liver peroxisome membranes (36). As increased message for Pxmp4 is associated with the NOD allele that confers weaker NKT cell stimulatory capacity, one possibility is that Pxmp4 contributes to the biosynthesis of a nonstimulatory etherphospholipid ligand for CD1d. Pxmp4 may be a component in a fatty acid degradation pathway that catabolizes stimulatory CD1d ligands. Peroxisomes play a critical role in the - and -oxidation of very long chain fatty acids. Pex19 associates with Pxmp4 during or immediately after its translation by free ribosomes. Peroxisomal membrane insertion of the Pxmp4-Pex19 complex is dependent on Pex3p.
Notes	This Human Peroxisomal membrane protein 4 (PXMP4) ELISA Kit employs a two-site sandwich ELISA to quantitate PXMP4 in samples. An antibody specific for PXMP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXMP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXMP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXMP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	11264

Applications	ELISA
Applications Notes	This Human Peroxisomal membrane protein 4 (PXMP4) ELISA Kit employs a two-site sandwich ELISA to quantitate PXMP4 in samples. An antibody specific for PXMP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXMP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXMP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXMP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703243	KTE61027	48 T	$339		Add to Cart
BHE13703243	KTE61027	96 T	$589		Add to Cart
BHE13703243	KTE61027	96 Tx5	$2629		Add to Cart
BHE13703243	KTE61027	96 Tx50	$24569		Add to Cart

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