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Home / ELISA Kit

Human Peroxiredoxin-5, mitochondrial (PRDX5) ELISA Kit

SKU : BHE13703395

Peroxiredoxin-5, mitochondrial is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes.
This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene.

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Mfr.No.	KTE61179
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Peroxiredoxin-5, mitochondrial (PRDX5) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDX5.
Alternative Names	PRDX5; ACR1; AOEB166; B166; MGC117264; MGC142283; MGC142285; PLP; PMP20; PRDX6; PRXV; SBBI10; Alu co-repressor 1; TPx type VI; antioxidant enzyme B166; liver tissue 2D-page spot 71B; peroxisomal antioxidant en
Detection Method	Colorimetric
Kit Components	Human Peroxiredoxin-5, mitochondrial microplate Human Peroxiredoxin-5, mitochondrial standard Human Peroxiredoxin-5, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Peroxiredoxin-5, mitochondrial (PRDX5) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRDX5. No significant cross-reactivity or interference between Human PRDX5 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Peroxiredoxin-5, mitochondrial is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene.
Notes	This Human Peroxiredoxin-5, mitochondrial (PRDX5) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDX5 in samples. An antibody specific for PRDX5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDX5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	25824

Applications	ELISA
Applications Notes	This Human Peroxiredoxin-5, mitochondrial (PRDX5) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDX5 in samples. An antibody specific for PRDX5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDX5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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