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Home / ELISA Kit

Human Peroxidasin-like protein (PXDNL) ELISA Kit

SKU : BHE13703247

PXDNL belongs to the peroxidase family. PXDNL is moderately expressed, only 28.6% of the average gene in this release, PXDNL contains 23 different gt-ag introns.
XPO subfamily. Dysfunction of mitochondria in nerve cells contributes to the neurodegeneration of PD. When cells are infected by the CMV virus, they become resistant to certain mitochondrial toxins that may be important in causing PD. CMV-infected cells produce very high levels of a protein called PXDNL about which very little is known. PXDNL is selectively found in substantia nigra neurons, increasing its levels will detoxify the free radicals produced by malfunctioning mitochondria and thereby protect these neurons. PXDNL may protect against these toxic processes and, if so, it may open the way for a new approach to slowing or halting the progression of the disease.

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Mfr.No.	KTE61031
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Peroxidasin-like protein (PXDNL) ELISA Kit employs a two-site sandwich ELISA to quantitate PXDNL.
Alternative Names	PXDNL; FLJ25471; VPO2; cardiac peroxidase; cardiovascular peroxidase 2; peroxidasin homolog-like
Detection Method	Colorimetric
Kit Components	Human Peroxidasin-like protein microplate Human Peroxidasin-like protein standard Human Peroxidasin-like protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Peroxidasin-like protein (PXDNL) ELISA Kit has high sensitivity and excellent specificity for detection of Human PXDNL. No significant cross-reactivity or interference between Human PXDNL and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PXDNL belongs to the peroxidase family. PXDNL is moderately expressed, only 28.6% of the average gene in this release, PXDNL contains 23 different gt-ag introns. XPO subfamily. Dysfunction of mitochondria in nerve cells contributes to the neurodegeneration of PD. When cells are infected by the CMV virus, they become resistant to certain mitochondrial toxins that may be important in causing PD. CMV-infected cells produce very high levels of a protein called PXDNL about which very little is known. PXDNL is selectively found in substantia nigra neurons, increasing its levels will detoxify the free radicals produced by malfunctioning mitochondria and thereby protect these neurons. PXDNL may protect against these toxic processes and, if so, it may open the way for a new approach to slowing or halting the progression of the disease.
Notes	This Human Peroxidasin-like protein (PXDNL) ELISA Kit employs a two-site sandwich ELISA to quantitate PXDNL in samples. An antibody specific for PXDNL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXDNL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXDNL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXDNL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	137902

Applications	ELISA
Applications Notes	This Human Peroxidasin-like protein (PXDNL) ELISA Kit employs a two-site sandwich ELISA to quantitate PXDNL in samples. An antibody specific for PXDNL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXDNL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXDNL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXDNL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703247	KTE61031	48 T	$339		Add to Cart
BHE13703247	KTE61031	96 T	$589		Add to Cart
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BHE13703247	KTE61031	96 Tx50	$24569		Add to Cart

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