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Home / ELISA Kit

Human Peroxidasin homolog (PXDN) ELISA Kit

SKU : BHE13703248

Drosophila peroxidasin is an extracellular matrix-associated peroxidase. It is expressed exclusively in hemocytes derived from head mesoderm at a very early stage of differentiation. Peroxidasin exists as a homotrimer with a unique hybrid structure that combines an enzymatically functional peroxidase domain with motifs that are typically found in extracellular matrix-associated proteins.
It is a secreted protein that contains a secretory recognition sequence at its N terminus. Peroxidasin catalyzes hydrogen peroxide-driven radioiodination, oxidations, and the formation of dityrosine in vitro. It is also thought to function in extracellular matrix consolidation, phagocytosis, and defense.

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Mfr.No.	KTE61032
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Peroxidasin homolog (PXDN) ELISA Kit employs a two-site sandwich ELISA to quantitate PXDN.
Alternative Names	PXDN; D2S448; D2S448E; KIAA0230; MG50; PRG2; PXN; VPO; p53-responsive gene 2; peroxidasin
Detection Method	Colorimetric
Kit Components	Human Peroxidasin homolog microplate Human Peroxidasin homolog standard Human Peroxidasin homolog detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Peroxidasin homolog (PXDN) ELISA Kit has high sensitivity and excellent specificity for detection of Human PXDN. No significant cross-reactivity or interference between Human PXDN and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Drosophila peroxidasin is an extracellular matrix-associated peroxidase. It is expressed exclusively in hemocytes derived from head mesoderm at a very early stage of differentiation. Peroxidasin exists as a homotrimer with a unique hybrid structure that combines an enzymatically functional peroxidase domain with motifs that are typically found in extracellular matrix-associated proteins. It is a secreted protein that contains a secretory recognition sequence at its N terminus. Peroxidasin catalyzes hydrogen peroxide-driven radioiodination, oxidations, and the formation of dityrosine in vitro. It is also thought to function in extracellular matrix consolidation, phagocytosis, and defense.
Notes	This Human Peroxidasin homolog (PXDN) ELISA Kit employs a two-site sandwich ELISA to quantitate PXDN in samples. An antibody specific for PXDN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXDN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXDN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXDN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7837

Applications	ELISA
Applications Notes	This Human Peroxidasin homolog (PXDN) ELISA Kit employs a two-site sandwich ELISA to quantitate PXDN in samples. An antibody specific for PXDN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXDN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXDN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXDN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703248	KTE61032	48 T	$339		Add to Cart
BHE13703248	KTE61032	96 T	$589		Add to Cart
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BHE13703248	KTE61032	96 Tx50	$24569		Add to Cart

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