Human Periplakin - PPL ELISA Kit

Mfr.No.

E5850Hu

Sensitivity

13.56ng/L

Detection Range

30-9000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

190 kDa paraneoplastic pemphigus antigen;195 kDa cornified envelope precursor protein;Periplakin;PPL

Molecular Weight

204747

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5850Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2038.8	81.96	4
Intra-Assay	2	18	3392.7	76.21	2.2
Intra-Assay	3	18	924.7	47.03	5.1

Background

Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.

Protocol

These standard curves of E5850Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.132	1.994	1.954
	1.855
2400ng/L	0.962	0.957	0.918
	0.953
1200ng/L	0.539	0.578	0.539
	0.618
600ng/L	0.172	0.194	0.155
	0.217
300ng/L	0.112	0.121	0.081
	0.13
0ng/L	0.043	0.04	0
	0.037

Gene ID	5493
UniProt ID	O60437

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107212	E5850Hu	48T	$320		Add to Cart
BHE12107212	E5850Hu	96T	$458		Add to Cart

Human Periplakin - PPL ELISA Kit

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