Human Periostin/OSF2 ELISA Kit PicoKine®

Mfr.No.

EK0985

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.010	0.052	0.100	0.166	0.302	0.582	0.978	1.804

Description

Human Periostin/OSF2 ELISA Kit PicoKine® (96 Tests). Quantitate Human POSTN in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Periostin;PN;Osteoblast-specific factor 2;OSF-2;POSTN;OSF2;

Molecular Weight

93314 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: N22-Q836

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Periostin

Subcellular Localization

Golgi apparatus . Secreted, extracellular space, extracellular matrix . Colocalizes with BMP1 in the Golgi. .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	188	1707	4824	180	1664	4867
Standard deviation	8.64	124.61	192.96	11.34	136.44	199.54
CV (%)	4.6%	7.3%	4%	8.2%	8.2%	4.1%

Protein Function

Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity). Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. .

Background

Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The Inteational Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	188	183	213	194	194	11.36	5.8%
Sample 2	1707	1857	1800	1793	1789	53.58	2.9%
Sample 3	4824	4787	4733	4555	4724	103.2	2.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	10631
Gene Name	POSTN
UniProt ID	Q15063
Protein Name	Periostin
Tissue Specificity	Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. .
Sequence	Contains 1 EMI domain.

Applications	ELISA
Research Area	Atherosclerosis,Cancer,Cardiogenesis,Cardiovascular,Cell Adhesion Molecules,Cell Type Markers,Cytoskeleton/ECM,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction,Structures,Tags & Cell Markers,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000748	EK0985	96 wells/kit	$399		Add to Cart

Human Periostin/OSF2 ELISA Kit PicoKine®

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