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Home / ELISA Kit

Human Period circadian protein homolog 1 (PER1) ELISA Kit

SKU : BHE13703464

PER1 is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. Alternative splicing has been observed in this gene; however, these variants have not been fully described.Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle.

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Mfr.No.	KTE61248
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Period circadian protein homolog 1 (PER1) ELISA Kit employs a two-site sandwich ELISA to quantitate PER1.
Alternative Names	PER1; MGC88021; PER; RIGUI; hPER; Period; drosophila; homolog of; circadian pacemaker protein RIGUI; period 1
Detection Method	Colorimetric
Kit Components	Human Period circadian protein homolog 1 microplate Human Period circadian protein homolog 1 standard Human Period circadian protein homolog 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Period circadian protein homolog 1 (PER1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PER1. No significant cross-reactivity or interference between Human PER1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PER1 is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. Alternative splicing has been observed in this gene; however, these variants have not been fully described.Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle.
Notes	This Human Period circadian protein homolog 1 (PER1) ELISA Kit employs a two-site sandwich ELISA to quantitate PER1 in samples. An antibody specific for PER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5187

Applications	ELISA
Applications Notes	This Human Period circadian protein homolog 1 (PER1) ELISA Kit employs a two-site sandwich ELISA to quantitate PER1 in samples. An antibody specific for PER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703464	KTE61248	48 T	$339		Add to Cart
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