Human Perinuclear anti-neutrophil cytoplasmic antibody,pANCA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04963h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Alternative Names

Perinuclear anti-neutrophil cytoplasmic antibody,pANCA

Recovery

The recovery of fish GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-104
EDTA plasma (n=4)	89	84-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	91-99
1:200	Average %	103
	Range %	99-107
1:400	Average %	98
	Range %	93-103
1:800	Average %	92
	Range %	87-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	pANCA

Storage	Store at 2-8°C. Please refer to protocol.

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