Human Pericentriolar Material 1 Protein

Mfr.No.

E5847Hu

Sensitivity

4.49ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

hPCM-1;PCM 1;PCM1;PCM-1;Pericentriolar material 1 protein

Molecular Weight

228544

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5847Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	486	28.27	5.8
Intra-Assay	2	18	272.6	18.13	6.7
Intra-Assay	3	18	151.9	7.94	5.2

Background

Required for centrosome assembly and function (PubMed: 12403812, PubMed: 15659651, PubMed: 16943179). Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (PubMed: 12403812, PubMed: 15659651). Required to anchor microtubules to the centrosome (PubMed: 12403812, PubMed: 15659651). Involved in the biogenesis of cilia (PubMed: 20551181, PubMed: 24121310).

Protocol

These standard curves of E5847Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.109	2.165	2.12
	2.222
600ng/L	1.843	1.792	1.747
	1.741
300ng/L	1.156	1.143	1.098
	1.13
150ng/L	0.679	0.654	0.609
	0.628
75ng/L	0.371	0.382	0.337
	0.394
0ng/L	0.043	0.045	0
	0.047

Gene ID	5108
UniProt ID	Q15154

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107209	E5847Hu	48T	$320		Add to Cart
BHE12107209	E5847Hu	96T	$458		Add to Cart

Human Pericentriolar Material 1 Protein - PCM1 ELISA Kit

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