Human Peptidase inhibitor 16(PI16) ELISA kit

Mfr.No.

CSB-EL017951HU

Sensitivity

0.156 ng/mL

Detection Range

0.312 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

peptidase inhibitor 16, CRISP9, DKFZp586B1817, MGC45378, MSMBBP, PSPBP, PSP94-binding protein, microseminoprotein, beta-binding protein, protease inhibitor 16

Recovery

The recovery of bovine IL18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-110
EDTA plasma (n=4)	97	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-92
1:2	Average %	97
	Range %	91-105
1:4	Average %	95
	Range %	92-99
1:8	Average %	93
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	3.011	2.978	2.995	2.900
1000	2.511	2.522	2.517	2.422
500	1.496	1.504	1.500	1.405
250	0.705	0.716	0.711	0.616
125	0.382	0.375	0.379	0.284
62.5	0.226	0.234	0.230	0.135
31.25	0.152	0.159	0.156	0.061
0	0.093	0.097	0.095	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q6UXB8

Applications	ELISA
Research Area	PI16

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503706	CSB-EL017951HU	96T	$695	Add to Cart
BHE10503706	CSB-EL017951HU	5x96T	$3336	Add to Cart
BHE10503706	CSB-EL017951HU	10x96T	$6602.50	Add to Cart

Human Peptidase inhibitor 16(PI16) ELISA kit

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