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Home / ELISA Kit

Human Pepsinogen A (PG-A) ELISA Kit

SKU : BHE13703455

Pepsinogen A is secreted exclusively by the chief cells of the gastric corpus. The levels of Pepsinogen I correlate directly with the active number of chief cells in this area. In cases of severe atrophic gastritis of the corpus region, the chief cells will be destroyed and the levels of Pepsinogen I will fall dramatically.Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. It consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41-amino acid segment from the amino end. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Samloff and Townes (1970) showed that the pepsinogen-5 derived from the stomach and excreted in the urine is absent in some persons. Family and population data supported the view that absence of PG-5 is recessive.

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Mfr.No.	KTE61239
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Pepsinogen A (PG-A) ELISA Kit employs a two-site sandwich ELISA to quantitate PG-A.
Alternative Names	PG-A
Detection Method	Colorimetric
Kit Components	Human Pepsinogen A microplate Human Pepsinogen A standard Human Pepsinogen A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Pepsinogen A (PG-A) ELISA Kit has high sensitivity and excellent specificity for detection of Human PG-A. No significant cross-reactivity or interference between Human PG-A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Pepsinogen A is secreted exclusively by the chief cells of the gastric corpus. The levels of Pepsinogen I correlate directly with the active number of chief cells in this area. In cases of severe atrophic gastritis of the corpus region, the chief cells will be destroyed and the levels of Pepsinogen I will fall dramatically.Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. It consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41-amino acid segment from the amino end. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Samloff and Townes (1970) showed that the pepsinogen-5 derived from the stomach and excreted in the urine is absent in some persons. Family and population data supported the view that absence of PG-5 is recessive.
Notes	This Human Pepsinogen A (PG-A) ELISA Kit employs a two-site sandwich ELISA to quantitate PG-A in samples. An antibody specific for PG-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPG-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PG-A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PG-A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Pepsinogen A (PG-A) ELISA Kit employs a two-site sandwich ELISA to quantitate PG-A in samples. An antibody specific for PG-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPG-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PG-A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PG-A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703455	KTE61239	48 T	$359		Add to Cart
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