Human Pepsin,PG ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08919h

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Pepsin,PG

Recovery

The recovery of mouse Col ? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-99
EDTA plasma (n=4)	100	96-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col ? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	89
	Range %	82-96
1:40	Average %	98
	Range %	94-102
1:80	Average %	97
	Range %	90-104
1:160	Average %	86
	Range %	82-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P0DJD8

Applications	ELISA
Research Area	PG

Storage	Store at 2-8°C. Please refer to protocol.

Reference	METHOD FOR EVALUATING SUITABILITY OF DUODENAL FLUID SAMPLE AS SAMPLE FOR DETECTING PANCREATIC FLUID-DERIVED COMPONENTS Asakura, Masanori .et al,United States Patent Application ,/ Relationship Between Salivary Pepsin Concentration and Esophageal Mucosal Integrity in Patients With Gastroesophageal Reflux Disease Li YW.et al,J Neurogastroenterol Motil.,Oct 30 The Relation of Reflux Finding Score and Reflux Symptom Index with Middle Ear Pepsin Level in Chronic Suppurative Otitis Media. Sriyana, Ayu Astria.et al,Journal of International Dental & Medical Research,/

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