Human PDK1([Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial) ELISA Kit

Mfr.No.

EH1189

Alternative Names

PDK1 ELISA Kit, PDPK1 ELISA Kit, (Pyruvate dehydrogenase (lipoamide)) kinase isozyme 1 ELISA Kit, mitochondrial ELISA Kit, mitochondrial pyruvate dehydrogenase ELISA Kit, lipoamide ELISA Kit, kinase isoenzyme 1 ELISA Kit, Pyruvate dehydrogenase kinase isoform 1 ELISA Kit, pyruvate dehydrogenase kinase ELISA Kit, isoenzyme 1 ELISA Kit, pyruvate dehydrogenase kinase ELISA Kit, isozyme 1 ELISA Kit, PKB kinase ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.313-20ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of PDK1 and the recovery rates were calculated by comparing the measured value to the expected amount of PDK1 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-105	97
EDTA plasma(n=5)	87-101	94
heparin plasma(n=5)	87-104	98

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	87-101%	88-105%	86-97%
EDTA plasma(n=5)	82-101%	82-100%	82-101%
heparin plasma(n=5)	84-99%	89-99%	84-98%

Sensitivity

< 0.188ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

PDK1/PDPK1/[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial/mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1/Pyruvate dehydrogenase kinase isoform 1/pyruvate dehydrogenase kinase, isoenzyme 1/pyruvate dehydrogenase kinase, isozyme 1/PKB kinase

Notes

For Research Use Only

UniProt ID	O15530
Antigen	PDK1

Applications	ELISA
Applications Notes	PDK1 ELISA Kit allows for the in vitro quantitative determination of PDK1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10806795	EH1189	96T	$400		Add to Cart

Human PDK1([Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial) ELISA Kit

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