Human PDGFAB(Platelet Derived Growth Factor AB) ELISA Kit

Mfr.No.

ELK1089

Alternative Names

PDGF-AB

Reactivity

Human

Detection Range

78.13-5000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant PDGFAB and the recovery rates were calculated by comparing the measured value to the expected amount of PDGFAB in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	80-94%	87%
Heparin plasma(n=5)	89-101%	95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGFAB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-103%	83-96%	95-102%	87-98%
EDTA plasma(n=5)	78-92%	96-105%	86-97%	85-101%
Heparin plasma(n=5)	85-94%	90-99%	82-101%	91-102%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
5000.00	1.952	1.868
2500.00	1.428	1.344
1250.00	1.067	0.983
625.00	0.756	0.672
312.50	0.435	0.351
156.25	0.279	0.195
78.13	0.157	0.073
0.00	0.084	0.000

Sensitivity

27 pg/mL

Limit Of Detection

5000 pg/mL

Antigen	PDGFAB
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDGFAB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDGFAB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDGFAB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDGFAB in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Cytokine;Tumor immunity;Hepatology;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15204218	ELK1089	48T	$265		Add to Cart
BHE15204218	ELK1089	96T	$376		Add to Cart

Human PDGFAB(Platelet Derived Growth Factor AB) ELISA Kit

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