Matrices listed below were spiked with certain level of PDE4D and the recovery rates were calculated by comparing the measured value to the expected amount of PDE4D in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
96
EDTA plasma(n=5)
86-105
97
heparin plasma(n=5)
90-98
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE4D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PDE4D ELISA Kit allows for the in vitro quantitative determination of PDE4D concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PDE4D (Phosphodiesterase 4D, cAMP Specific) ELISA Kit