Human PDE4A(cAMP-specific 3',5'-cyclic phosphodiesterase 4A) ELISA Kit

Mfr.No.

EH10975

Description

Human PDE4A(cAMP-specific 3',5'-cyclic phosphodiesterase 4A) ELISA Kit

Alternative Names

cAMP-specific 3',5'-cyclic phosphodiesterase 4A ELISA Kit, DPDE2 ELISA Kit, PDE46 ELISA Kit, PDE4A ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

31.25-2000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of PDE4A and the recovery rates were calculated by comparing the measured value to the expected amount of PDE4A in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	87-104	94
EDTA plasma(n=5)	88-101	93
heparin plasma(n=5)	88-94	91

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE4A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	88-104%	89-102%	87-104%
EDTA plasma(n=5)	85-100%	84-96%	84-101%
heparin plasma(n=5)	83-92%	86-100%	85-92%

Sensitivity

< 18.75pg/ml

Notes

For Research Use Only

UniProt ID	P27815

Applications	ELISA
Applications Notes	PDE4AELISA Kit allows for the in vitro quantitative determination of PDE4A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10808715	EH10975	96T	$400		Add to Cart

Human PDE4A(cAMP-specific 3 ,5 -cyclic phosphodiesterase 4A)ELISA Kit

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