Matrices listed below were spiked with certain level of PCK2 and the recovery rates were calculated by comparing the measured value to the expected amount of PCK2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-101
97
EDTA plasma(n=5)
85-99
92
heparin plasma(n=5)
90-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PCK2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PCK2 ELISA Kit allows for the in vitro quantitative determination of PCK2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PCK2(Phosphoenolpyruvate carboxykinase [GTP], mitochondrial) ELISA Kit