Human Paxillin - PAX ELISA Kit

Mfr.No.

E0685Hu

Sensitivity

0.01ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Paxillin (also known as PXN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Paxillin;PXN

Molecular Weight

64505

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0685Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.9	0.48	6.1
Intra-Assay	2	18	2	0.08	3.8
Intra-Assay	3	18	3.4	0.16	4.7

Background

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Protocol

These standard curves of E0685Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.12	2.107	2.044
	2.093
10ng/ml	1.951	1.908	1.845
	1.865
5ng/ml	1.294	1.233	1.17
	1.171
2.5ng/ml	0.639	0.683	0.62
	0.726
1.25ng/ml	0.386	0.374	0.311
	0.363
0ng/ml	0.066	0.063	0
	0.06

Gene ID	5829
UniProt ID	P49023

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102367	E0685Hu	96T	$458		Add to Cart
BHE12102367	E0685Hu	48T	$320		Add to Cart

Human Paxillin - PAX ELISA Kit

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