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Human Patatin-like phospholipase domain-containing protein 3 (PNPLA3/ADPN/C22orf20) ELISA Kit

SKU : BHE13703415

PNPLA3 is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes.
The glucose-induced increase in Adpn expression could be countered by agents known to raise intracellular cAMP. In vivo, Adpn mRNA was high in white and brown adipose tissue from fed mice, but it dropped to barely detectable levels after 19 hours of fast. Refeeding for 8 hours reversed the drop in Adpn mRNA. Fas and Srebp1c mRNA exhibited a similar pattern of expression. Just after a feeding period, Zucker fa/fa obese rats showed 30- to 50-fold elevation of Adpn mRNA relative to congenic lean controls.

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Mfr.No.	KTE61199
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Patatin-like phospholipase domain-containing protein 3 (PNPLA3/ADPN/C22orf20) ELISA Kit employs a two-site sandwich ELISA to quantitate PNPLA3.
Alternative Names	PNPLA3; RP4-796I17.1; ADPN; C22orf20; iPLA(2)epsilon; adiponutrin
Detection Method	Colorimetric
Kit Components	Human Patatin-like phospholipase domain-containing protein 3 microplate Human Patatin-like phospholipase domain-containing protein 3 standard Human Patatin-like phospholipase domain-containing protein 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Patatin-like phospholipase domain-containing protein 3 (PNPLA3/ADPN/C22orf20) ELISA Kit has high sensitivity and excellent specificity for detection of Human PNPLA3. No significant cross-reactivity or interference between Human PNPLA3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PNPLA3 is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. The glucose-induced increase in Adpn expression could be countered by agents known to raise intracellular cAMP. In vivo, Adpn mRNA was high in white and brown adipose tissue from fed mice, but it dropped to barely detectable levels after 19 hours of fast. Refeeding for 8 hours reversed the drop in Adpn mRNA. Fas and Srebp1c mRNA exhibited a similar pattern of expression. Just after a feeding period, Zucker fa/fa obese rats showed 30- to 50-fold elevation of Adpn mRNA relative to congenic lean controls.
Notes	This Human Patatin-like phospholipase domain-containing protein 3 (PNPLA3/ADPN/C22orf20) ELISA Kit employs a two-site sandwich ELISA to quantitate PNPLA3 in samples. An antibody specific for PNPLA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNPLA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNPLA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNPLA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	80339

Applications	ELISA
Applications Notes	This Human Patatin-like phospholipase domain-containing protein 3 (PNPLA3/ADPN/C22orf20) ELISA Kit employs a two-site sandwich ELISA to quantitate PNPLA3 in samples. An antibody specific for PNPLA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNPLA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNPLA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNPLA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703415	KTE61199	48 T	$359		Add to Cart
BHE13703415	KTE61199	96 T	$619		Add to Cart
BHE13703415	KTE61199	96 Tx5	$2809		Add to Cart
BHE13703415	KTE61199	96 Tx50	$26209		Add to Cart

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